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procedure is recommended by the Office of Radiation, Chemical and
Biological Safety for the decontamination of biohazardous waste.
The following materials need to be autoclaved prior to disposal:
- Culture and
stocks of infectious agents
- Culture dishes
and related devices
solid items such as paper towels, cloth and plastic pipette tips,
pipettes and vials, petri dishes and gloves.
live and attenuated vaccines
2. Items should
be autoclaved in MSU approved autoclave bags and a rigid secondary
3. Follow the
guidelines set by the posted autoclave parameter signs when setting
Add one cup of water to each bag of solid waste and keep the bags
OPEN--steam can not penetrate closed bags.
5. To prevent
spills and accidents, be sure that the exhaust setting is appropriate
for the type of material you are autoclaving.
Use FAST exhaust for solid items (solid waste, instruments) and
SLOW exhaust for liquids and liquid wastes.
6. After the
cycle is completed, let the bag cool for several minuets before
removing it from the autoclave.
Securely close orange autoclave bag.
7. Place treated
autoclave bags into opaque (non see-through) black bag and close
them securely before disposing. To assure that the black bag does
not rupture, do not put multiple orange bags in a single black bag.
8. Don't forget
to mark your Autoclave Waste Tracking
Sheet for each bag of biohazardous waste that you treat.
dependable and skilled operators
Autoclave Procedures Table of Contents