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THE ORCBS > Biological Safety > Programs & Guidelines > Autoclave Procedures > AUTOCLAVE PROCEDURES Effective Sterilization: Proper loading of the Unit
While clean and contaminated items may be sterilized in the same autoclave, do not mix them together during the same cycle-they require different heat exposure times. Never place sharps (e.g. syringes with needles) in a biohazard bag. All sharps need to be disposed of in sharps containers, which will be incinerated. 
Autoclave Procedures Table of Contents |
To
effectively sterilize biohazardous wastes, it is important to properly
load the autoclave. Adding one cup of water to solid wastes creates
additional steam that drives residual air from the bag. It is also
important to allow the steam to circulate freely throughout the
chamber-do not overload the chamber with bags that are too large
for the capacity of your autoclave.
