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AUTOCLAVE PROCEDURES

Effective Sterilization: Approved and properly operating sterilizer

The ability of autoclaves on campus to sterilize biohazardous waste is determined though yearly testing by the Office of Radiation, Chemical and Biological Safety. These tests determine the time, pressure and temperature needed to safely decontaminate waste in each autoclave through the use of a biological indicator which uses spores of Bacillus stearothermophilus, which is rendered unviable at 250 ° F (121 ° C). For the test, ampoules of B. stearothermophilus are autoclaved along with a load of waste. Upon completion of the cycle, the ampoules are incubated for 48 hours and then observed for any sign of growth with would indicate that autoclave is not sterilizing properly. If growth occurs, the autoclaves are re-tested until a cycle time that allows for decontamination is found. The results are posted on nearby autoclave parameter signs. Any questions about this procedure should be directed to the Biological Safety Graduate Assistant, 432-5019.

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