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THE ORCBS > Biological Safety > Programs & Guidelines > Autoclave Procedures > AUTOCLAVE PROCEDURES Unloading the Autoclave Safely
Use special caution when autoclaving containers that may have become pressurized. Never autoclave a sealed container of liquids as this may result in an explosion of super-heated liquid during the cycle or when the container is opened. 
Autoclave Procedures Table of Contents |
When
the cycle is complete, wait until the chamber pressure gauge reads
zero before attempting to open the autoclave. A waiting period of
several minutes before removing the bag from the autoclave will
allow the chamber and any residual liquids to cool, significantly
reducing your chances of getting burned. Also be aware of molten
agar that may have collected in the secondary container during the
cycle.
