THE ORCBS > Biological Safety > Programs & Guidelines > Biosafety Manual >

TABLE OF CONTENTS

• Foreward
• Biohazard Definition
• Rules, Regulations & Guidelines
• Risk Assessment
  • Agent Hazards
  • Laboratory Procedure Hazards
• Biological Safety and Biosafety Levels
  • General Laboratory Practices
  • Routes of Infection
  • Access
  • Personal Protective Equipment
    • Eye and Face Protection
    • Laboratory Clothing
    • Gloves
    • Respirators
  • Laundry
  • Housekeeping
  • Training
  • Food and Drink Policy
  • Health and Medical Surveillance
  • Inventory Log
• Biological Safety Level 1 Laboratories
  • Laboratory Design and Facilities
  • Standard Microbiological Practices
  • Special Practices
  • Safety Equipment
• Biological Safety Level 2 Laboratories
  • Laboratory Design and Facilities
  • Standard Microbiological Practices
  • Special Practices
  • Safety Equipment
• Biological Safety Level 3 Laboratories
  • Laboratory Design and Facilities
  • Standard Microbiological Practices
  • Special Practices
  • Safety Equipment
• Laboratory Animal Facilities
  • Animal Facility-Biosafety Level 1
    • Standard Microbiological Practices
    • Safety Equipment
    • Laboratory Facilities
  • Animal Facility- Biosafety Level 2
    • Standard Microbiological Practices
    • Special Practices
    • Safety Equipment
    • Laboratory Facilities
  • Animal Facility- Biosafety Level 3
    • Standard Microbiological Practices
    • Special Practices
    • Safety Equipment
    • Laboratory Facilities
  • Invertebrates
• Plant Biosafety Levels
  • Plant Biosafety Level 1
    • Standard Practices
    • Facilities
  • Plant Biosafety Level 2
    • Standard Practices
    • Facilities
• Laboratory Biosecurity
• Safety Equipment
  • Biological Safety Cabinets
    • Class I Biological Safety Cabinet
    • Class II Biological Safety Cabinet
    • Class III Biological Safety Cabinet
    • Selection of a Biological Safety Cabinet
    • Using Biological Safety Cabinets in the Laboratory
• Pipetting Aids
• Homogenizers, Shakers, Blenders and Sonicators
• Disposable Transfer Loops, Needles and Cell Spreaders
• Recommended Work Practices
  • Autoclaves
  • Flow Cytometers
  • Pipettes and Pipetting Aids
  • Sharps
  • Cryostats
  • Centrifuge Equipment
  • Safety Blenders
  • Lyophilizers and Ampoules
  • Loop Sterilizers and Bunsen Burners
• Guidelines for Working with Tissue Culture/Cell Lines
• Guidelines for Preventing the Transmission of Tuberculosis
• Guidelines for Clinical Laboratories
• Guidelines for Prion Use
• Guidelines Regarding Select Agents
• Guidelines for Handling Exempt Strains of Select Agents
• Guidelines for the Use of Exempt Levels of Select Agent Toxins
• Decontamination
  • Cleaning Laboratory Materials
  • Ways to Decontaminate
    • Heat
    • Liquid Chemicals Used as Disinfectants
    • Vapors and Gases
    • Radiation
    • Incineration
• Biohazardous Waste
  • General Labeling, Packaging and Disposal Procedures
  • Waste Specific Procedures for BSL-1 and 2
  • Waste Specific Procedures for Biosafety Level 3
  • Pathological Waste
  • Animal Waste
  • Department or Facility Specific Waste Procedures
• Biohazard Spill Cleanup Procedures
  • Basic Biological Spill Kit Contents
  • Spills with no Broken Glass/Sharps
  • Spills Involving Broken Glass/Sharps
  • Spills Inside the Biological Safety Cabinet
  • Spills Inside a Centrifuge
  • Spills During Transport
  • Spill Kit Maintenance
• Handwashing and Hand Decontamination
• Introduction to the Transport of Biological Materials
  • Transporting Biological Materials on Campus
  • Shipping of Biological Materials to an Off Campus Destination
• Use of Animals in Research
• Use of Human Subjects and Materials in Research
• Biosafety and Recombinant DNA Technology
  • NIH Recombinant DNA Review Categories
  • Review Process Overview
  • Responsibilities of the Principal Investigator for Recombinant DNA Research
• Appendices
  • Appendix A- Biosafety Resources
  • Appendix B- DHHS/USDA Select Agents List
  • Appendix C- Risk Assessment Form
  • Appendix D- Examples of RG-2, RG-3 and RG-4 Agents
  • Appendix E- Equipment Release Form
  • Appendix F- Exposure Response Procedures
  • Appendix G- Lab and Animal Biosafety Level Summaries
  • Appendix H- Example Inventory Log
  • Appendix I- Biosafety Practices for Handling Prions
  • Appendix J- Requirements for Handling Exempt Strains of Select Agents
  • Appendix K- Site-Specific Training Checklist