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THE ORCBS > Biological Safety > Programs & Guidelines > Biosafety Manual >
Class III Biological Safety Cabinet This type provides the highest level of personnel protection and in used for Risk Group 4 agents. All penetrations are sealed "gas tight." Supply air is HEPA-filtered and exhaust air passes through two HEPA filters. Airflow is maintained by a dedicated exhaust system exterior to the cabinet, which keeps the cabinet interior under negative pressure. Access to the work surface is by means of heavy duty rubber gloves, which are attached to ports in the cabinet. The Class III BSC should have an attached pass-through box that can be sterilized and is equipped with a HEPA-filtered exhaust. The Class III cabinet may be connected to a double-door autoclave used to decontaminate all materials entering to exiting the cabinet. Several glove boxes can be joined together to extend the work surface. Class III BSCs are suitable for work in Biosafety Level 3 and 4 laboratories. |
Safety Equipment: Biological Ssafety Cabinets (BSCs)
