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Recommended Work Practices: Loop Sterilizers and Bunsen Burners

Sterilization of inoculating loops or needles in an open flame generates small particle aerosols which may contain viable microorganisms. The use of a shielded electric incinerator or hot bead sterilizers minimizes aerosol production during loop sterilization. Alternatively, disposable plastic loops and needles may be used for culture work.

Continuous flame gas burners should not be used in BSCs. These burners can produce turbulence which disturbs the protective airflow patterns of the cabinet. Additionally, the heat produced by the continuous flame may damage the HEPA filter.

Guidelines for Working with Tissue Culture/Cell Lines

Biosafety Manual Table of Contents